Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6818..7311 Replicon plasmid p1540-1
Accession NZ_CP019052
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag BVL39_RS25585 Protein ID WP_001230709.1
Coordinates 6818..7072 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag BVL39_RS25590 Protein ID WP_000151456.1
Coordinates 7069..7311 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS25550 3123..3284 + 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -
BVL39_RS25555 3434..4030 + 597 WP_000125165.1 hypothetical protein -
BVL39_RS29285 4048..4332 - 285 WP_001162371.1 hypothetical protein -
BVL39_RS25565 4406..5071 - 666 WP_000532148.1 P-loop NTPase -
BVL39_RS25575 5660..6175 + 516 WP_001025393.1 J domain-containing protein -
BVL39_RS25580 6279..6821 + 543 WP_001083904.1 hypothetical protein -
BVL39_RS25585 6818..7072 + 255 WP_001230709.1 hypothetical protein Toxin
BVL39_RS25590 7069..7311 + 243 WP_000151456.1 hypothetical protein Antitoxin
BVL39_RS25595 7448..7780 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
BVL39_RS25600 7827..8702 - 876 WP_015387340.1 class A extended-spectrum beta-lactamase CTX-M-55 -
BVL39_RS25605 8955..10217 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
BVL39_RS25615 10976..11305 + 330 WP_000855120.1 hypothetical protein -
BVL39_RS25625 11886..12209 + 324 WP_000427403.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-55 / mcr-1.1 - 1..65533 65533
- flank IS/Tn blaCTX-M-55 - 7827..10217 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T71635 WP_001230709.1 NZ_CP019052:6818-7072 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T71635 NZ_CP019052:6818-7072 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT71635 WP_000151456.1 NZ_CP019052:7069-7311 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT71635 NZ_CP019052:7069-7311 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References