Detailed information of TA system TA071634

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4562499..4563333
• Replicon: chromosome
• Accession: NZ_CP019051
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: W0S379
• Locus tag: BVL39_RS23595
• Protein ID: WP_000854808.1
• Coordinates: 4562499..4562876 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J4HRK6
• Locus tag: BVL39_RS23600
• Protein ID: WP_001354276.1
• Coordinates: 4562965..4563333 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS23565	4559192..4559896	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
BVL39_RS23570	4560181..4560399	+	219	WP_001040187.1	translation initiation factor IF-1	-
BVL39_RS23580	4560878..4561720	-	843	WP_072643849.1	DUF4942 domain-containing protein	-
BVL39_RS23585	4561805..4562002	-	198	WP_072643848.1	DUF957 domain-containing protein	-
BVL39_RS23590	4562014..4562502	-	489	WP_039022338.1	hypothetical protein	-
BVL39_RS23595	4562499..4562876	-	378	WP_000854808.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BVL39_RS23600	4562965..4563333	-	369	WP_001354276.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BVL39_RS23605	4563383..4564027	-	645	WP_072643847.1	antitoxin of toxin-antitoxin stability system	-
BVL39_RS23610	4564046..4564267	-	222	WP_000692312.1	DUF987 domain-containing protein	-
BVL39_RS23615	4564336..4564812	-	477	WP_001186725.1	RadC family protein	-
BVL39_RS23620	4564828..4565313	-	486	WP_000214415.1	antirestriction protein	-
BVL39_RS23625	4565405..4566223	-	819	WP_072643846.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13909.93 Da
• Isoelectric Point: 7.9085

>T71634 WP_000854808.1 NZ_CP019051:c4562876-4562499 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T71634 NZ_CP019051:c4562876-4562499 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13649.47 Da
• Isoelectric Point: 7.0261

>AT71634 WP_001354276.1 NZ_CP019051:c4563333-4562965 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT71634 NZ_CP019051:c4563333-4562965 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGTACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: W0S379

Antitoxin

• AlphaFold DB: A0A2J4HRK6