Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4028231..4028869 Replicon chromosome
Accession NZ_CP019051
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BVL39_RS20855 Protein ID WP_000813794.1
Coordinates 4028693..4028869 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BVL39_RS20850 Protein ID WP_001270285.1
Coordinates 4028231..4028647 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS20830 4023383..4024324 - 942 WP_001251313.1 ABC transporter permease -
BVL39_RS20835 4024325..4025338 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
BVL39_RS20840 4025356..4026501 - 1146 WP_000034363.1 ABC transporter substrate-binding protein -
BVL39_RS20845 4026746..4028152 - 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
BVL39_RS20850 4028231..4028647 - 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BVL39_RS20855 4028693..4028869 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BVL39_RS20860 4029091..4029321 + 231 WP_000494244.1 YncJ family protein -
BVL39_RS20865 4029413..4031374 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BVL39_RS20870 4031447..4031983 - 537 WP_000429153.1 DNA-binding transcriptional regulator SutR -
BVL39_RS20875 4032036..4033250 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T71633 WP_000813794.1 NZ_CP019051:c4028869-4028693 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T71633 NZ_CP019051:c4028869-4028693 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT71633 WP_001270285.1 NZ_CP019051:c4028647-4028231 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT71633 NZ_CP019051:c4028647-4028231 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References