Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2791151..2791776 Replicon chromosome
Accession NZ_CP019051
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag BVL39_RS14355 Protein ID WP_000911329.1
Coordinates 2791378..2791776 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag BVL39_RS14350 Protein ID WP_000450524.1
Coordinates 2791151..2791378 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS14325 2786953..2787423 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
BVL39_RS14330 2787423..2787995 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
BVL39_RS14335 2788141..2789019 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
BVL39_RS14340 2789036..2790070 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
BVL39_RS14345 2790283..2790996 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
BVL39_RS14350 2791151..2791378 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
BVL39_RS14355 2791378..2791776 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
BVL39_RS14360 2791923..2792786 + 864 WP_001267498.1 neutral zinc metallopeptidase -
BVL39_RS14365 2792801..2794816 + 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
BVL39_RS14370 2794890..2795588 + 699 WP_000679823.1 esterase -
BVL39_RS14375 2795698..2795898 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T71622 WP_000911329.1 NZ_CP019051:2791378-2791776 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T71622 NZ_CP019051:2791378-2791776 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT71622 WP_000450524.1 NZ_CP019051:2791151-2791378 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT71622 NZ_CP019051:2791151-2791378 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References