Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2361339..2361993 Replicon chromosome
Accession NZ_CP019051
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag BVL39_RS12215 Protein ID WP_000244772.1
Coordinates 2361586..2361993 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BVL39_RS12210 Protein ID WP_000354046.1
Coordinates 2361339..2361605 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS12185 2356508..2357251 + 744 WP_101973104.1 SDR family oxidoreductase -
BVL39_RS12190 2357308..2358741 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
BVL39_RS12195 2358786..2359097 + 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
BVL39_RS12200 2359261..2359920 + 660 WP_000250274.1 hemolysin III family protein -
BVL39_RS12205 2360116..2361096 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
BVL39_RS12210 2361339..2361605 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BVL39_RS12215 2361586..2361993 + 408 WP_000244772.1 protein YgfX Toxin
BVL39_RS12220 2362033..2362554 - 522 WP_001055874.1 flavodoxin FldB -
BVL39_RS12225 2362666..2363562 + 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
BVL39_RS12230 2363587..2364297 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BVL39_RS12235 2364303..2366036 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T71620 WP_000244772.1 NZ_CP019051:2361586-2361993 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T71620 NZ_CP019051:2361586-2361993 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT71620 WP_000354046.1 NZ_CP019051:2361339-2361605 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT71620 NZ_CP019051:2361339-2361605 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References