Detailed information of TA system TA071612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 141839..142676
• Replicon: chromosome
• Accession: NZ_CP019051
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: BVL39_RS00690
• Protein ID: WP_000227784.1
• Coordinates: 142134..142676 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BVL39_RS00685
• Protein ID: WP_001297137.1
• Coordinates: 141839..142150 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS00660	136859..137806	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
BVL39_RS00665	137828..139819	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BVL39_RS00670	139809..140423	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BVL39_RS00675	140423..140752	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BVL39_RS00680	140764..141654	+	891	WP_000971332.1	heme o synthase	-
BVL39_RS00685	141839..142150	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BVL39_RS00690	142134..142676	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
BVL39_RS00695	142732..143667	-	936	WP_001297127.1	sel1 repeat family protein	-
BVL39_RS00700	144075..145439	+	1365	WP_072643898.1	MFS transporter	-
BVL39_RS00705	145567..146058	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
BVL39_RS00710	146226..147137	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T71612 WP_000227784.1 NZ_CP019051:142134-142676 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T71612 NZ_CP019051:142134-142676 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT71612 WP_001297137.1 NZ_CP019051:141839-142150 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT71612 NZ_CP019051:141839-142150 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3