Detailed information of TA system TA071610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 187213..187883
• Replicon: plasmid pRJA166b
• Accession: NZ_CP019049
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: RJA_RS28810
• Protein ID: WP_087525970.1
• Coordinates: 187440..187883 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: RJA_RS28805
• Protein ID: WP_015874976.1
• Coordinates: 187213..187443 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJA_RS28775	183436..184335	-	900	WP_004225022.1	nucleotide-binding protein	-
RJA_RS28780	184325..184615	-	291	WP_004213078.1	hypothetical protein	-
RJA_RS28785	184967..185173	+	207	WP_004213077.1	hypothetical protein	-
RJA_RS28790	185163..185456	-	294	WP_004213076.1	hypothetical protein	-
RJA_RS28795	185472..186605	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
RJA_RS28805	187213..187443	+	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RJA_RS28810	187440..187883	+	444	WP_087525970.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJA_RS28815	187897..188313	-	417	Protein_200	restriction endonuclease	-
RJA_RS28820	188317..188610	-	294	Protein_201	transposase	-
RJA_RS30605	189027..189664	+	638	Protein_202	response regulator	-
RJA_RS28840	190179..190585	-	407	Protein_203	GAF domain-containing protein	-
RJA_RS28850	190676..191596	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
RJA_RS28855	191645..192136	-	492	WP_011251327.1	DM13 domain-containing protein	-
RJA_RS28860	192199..192474	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC	1..228613	228613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15964.66 Da
• Isoelectric Point: 9.3642

>T71610 WP_087525970.1 NZ_CP019049:187440-187883 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T71610 NZ_CP019049:187440-187883 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT71610 WP_015874976.1 NZ_CP019049:187213-187443 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT71610 NZ_CP019049:187213-187443 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A4GZE4