Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 10935..11845 Replicon plasmid pRJA166b
Accession NZ_CP019049
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag RJA_RS27810 Protein ID WP_004026354.1
Coordinates 11375..11845 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag RJA_RS27805 Protein ID WP_004026357.1
Coordinates 10935..11378 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJA_RS27770 5953..6474 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
RJA_RS29965 6471..7424 + 954 Protein_8 fec operon regulator FecR -
RJA_RS27785 7511..9637 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
RJA_RS27790 9712..9867 + 156 WP_004213642.1 hypothetical protein -
RJA_RS27795 10012..10233 + 222 WP_004213643.1 DUF2188 domain-containing protein -
RJA_RS27800 10331..10834 + 504 Protein_12 DUF4113 domain-containing protein -
RJA_RS27805 10935..11378 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
RJA_RS27810 11375..11845 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
RJA_RS27815 11994..12691 + 698 Protein_15 IS1 family transposase -
RJA_RS27820 14077..15026 - 950 Protein_16 DUF1173 family protein -
RJA_RS27825 15204..15698 + 495 WP_004212794.1 thermonuclease family protein -
RJA_RS27830 16035..16433 + 399 WP_004212796.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC 1..228613 228613
- flank IS/Tn - - 12188..12691 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T71608 WP_004026354.1 NZ_CP019049:11375-11845 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T71608 NZ_CP019049:11375-11845 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT71608 WP_004026357.1 NZ_CP019049:10935-11378 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT71608 NZ_CP019049:10935-11378 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References