Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4046328..4046947 Replicon chromosome
Accession NZ_CP019047
Organism Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag RJA_RS20050 Protein ID WP_002892050.1
Coordinates 4046729..4046947 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag RJA_RS20045 Protein ID WP_002892066.1
Coordinates 4046328..4046702 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJA_RS20035 4041480..4042673 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RJA_RS20040 4042696..4045842 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
RJA_RS20045 4046328..4046702 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
RJA_RS20050 4046729..4046947 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
RJA_RS20055 4047106..4047672 + 567 WP_002892030.1 maltose O-acetyltransferase -
RJA_RS30445 4047644..4047784 - 141 WP_004147370.1 hypothetical protein -
RJA_RS20060 4047805..4048275 + 471 WP_002892026.1 YlaC family protein -
RJA_RS20065 4048250..4049707 - 1458 WP_041937768.1 PLP-dependent aminotransferase family protein -
RJA_RS20070 4049808..4050506 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
RJA_RS20075 4050503..4050643 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
RJA_RS20080 4050643..4050906 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T71603 WP_002892050.1 NZ_CP019047:4046729-4046947 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T71603 NZ_CP019047:4046729-4046947 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT71603 WP_002892066.1 NZ_CP019047:4046328-4046702 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT71603 NZ_CP019047:4046328-4046702 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References