Detailed information of TA system TA071593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1844955..1845598
• Replicon: chromosome
• Accession: NZ_CP019047
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain RJA166

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: RJA_RS09160
• Protein ID: WP_015874977.1
• Coordinates: 1844955..1845371 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: RJA_RS09165
• Protein ID: WP_015874976.1
• Coordinates: 1845368..1845598 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJA_RS09135	1840358..1841473	-	1116	WP_032447702.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
RJA_RS09150	1842345..1844519	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
RJA_RS09155	1844674..1844919	-	246	WP_032447699.1	hypothetical protein	-
RJA_RS09160	1844955..1845371	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJA_RS09165	1845368..1845598	-	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RJA_RS09175	1846041..1846421	+	381	WP_015874975.1	YrzE family protein	-
RJA_RS09185	1846558..1847511	+	954	Protein_1760	IS21 family transposase	-
RJA_RS09190	1847606..1848332	+	727	Protein_1761	IS21-like element IS100kyp family helper ATPase IstB	-
RJA_RS09195	1848376..1848489	-	114	WP_015874970.1	hypothetical protein	-
RJA_RS09200	1848732..1848941	-	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
RJA_RS09205	1848931..1849512	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
RJA_RS09210	1849497..1849679	-	183	WP_072001695.1	hypothetical protein	-
RJA_RS09215	1849701..1849886	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iroD / iroC / iroB / iroN / fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP	1806260..1876393	70133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T71593 WP_015874977.1 NZ_CP019047:c1845371-1844955 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T71593 NZ_CP019047:c1845371-1844955 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCACCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT71593 WP_015874976.1 NZ_CP019047:c1845598-1845368 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT71593 NZ_CP019047:c1845598-1845368 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4