71582

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1465278..1465815 Replicon chromosome
Accession NZ_CP019043
Organism Burkholderia pseudomallei strain 14M0960418

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BHT10_RS29070 Protein ID WP_004549731.1
Coordinates 1465278..1465547 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BHT10_RS29075 Protein ID WP_004525420.1
Coordinates 1465531..1465815 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT10_RS29030 1461022..1462062 - 1041 WP_197970191.1 ImmA/IrrE family metallo-endopeptidase -
BHT10_RS29040 1462278..1462562 - 285 WP_009961460.1 type II toxin-antitoxin system HigB family toxin -
BHT10_RS38210 1463466..1463708 - 243 WP_009961458.1 hypothetical protein -
BHT10_RS38215 1463756..1463914 - 159 WP_004549802.1 hypothetical protein -
BHT10_RS29050 1463911..1464153 - 243 WP_004545584.1 hypothetical protein -
BHT10_RS29060 1464291..1464422 - 132 WP_009923452.1 bacteriophage protein Gp48 -
BHT10_RS29065 1464432..1464707 - 276 WP_025988352.1 hypothetical protein -
BHT10_RS29070 1465278..1465547 + 270 WP_004549731.1 BrnT family toxin Toxin
BHT10_RS29075 1465531..1465815 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BHT10_RS29090 1466462..1466845 + 384 Protein_1207 DDE-type integrase/transposase/recombinase -
BHT10_RS29095 1466808..1467236 + 429 Protein_1208 IS110 family transposase -
BHT10_RS29100 1467463..1467579 - 117 Protein_1209 IS3 family transposase -
BHT10_RS29105 1467826..1469286 + 1461 WP_011326543.1 metallophosphoesterase -
BHT10_RS29110 1469445..1470707 + 1263 WP_004537187.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1447310..1466845 19535


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T71582 WP_004549731.1 NZ_CP019043:1465278-1465547 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T71582 NZ_CP019043:1465278-1465547 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT71582 WP_004525420.1 NZ_CP019043:1465531-1465815 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT71582 NZ_CP019043:1465531-1465815 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References