Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511817..512454 Replicon chromosome
Accession NZ_CP019040
Organism Bacillus velezensis strain GH1-13

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BVH55_RS02950 Protein ID WP_003156187.1
Coordinates 512104..512454 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BVH55_RS02945 Protein ID WP_003156188.1
Coordinates 511817..512098 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVH55_RS02925 508182..508781 - 600 WP_077721836.1 rhomboid family intramembrane serine protease -
BVH55_RS02930 508874..509239 + 366 WP_003156192.1 holo-ACP synthase -
BVH55_RS02935 509404..510411 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
BVH55_RS02940 510528..511697 + 1170 WP_077721837.1 alanine racemase -
BVH55_RS02945 511817..512098 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BVH55_RS02950 512104..512454 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BVH55_RS02955 512572..513393 + 822 WP_003156182.1 STAS domain-containing protein -
BVH55_RS02960 513398..513763 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BVH55_RS02965 513766..514167 + 402 WP_003156178.1 anti-sigma regulatory factor -
BVH55_RS02970 514179..515186 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
BVH55_RS02975 515250..515579 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BVH55_RS02980 515576..516058 + 483 WP_003156173.1 anti-sigma B factor RsbW -
BVH55_RS02985 516024..516812 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BVH55_RS02990 516812..517414 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T71578 WP_003156187.1 NZ_CP019040:512104-512454 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T71578 NZ_CP019040:512104-512454 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT71578 WP_003156188.1 NZ_CP019040:511817-512098 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT71578 NZ_CP019040:511817-512098 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACGACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References