Detailed information of TA system TA071573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 18706..19904
• Replicon: plasmid pSNU175-4
• Accession: NZ_CP019033
• Organism: Limosilactobacillus fermentum strain SNUV175

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: BUW47_RS11505
• Protein ID: WP_075667810.1
• Coordinates: 18706..19629 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: V5E259
• Locus tag: BUW47_RS11510
• Protein ID: WP_007124950.1
• Coordinates: 19632..19904 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUW47_RS11470	14210..14506	-	297	WP_113532503.1	WXG100 family type VII secretion target	-
BUW47_RS11685	14724..15707	+	984	WP_081378719.1	CHAP domain-containing protein	-
BUW47_RS11490	15843..17906	-	2064	WP_075667808.1	MobA/MobL family protein	-
BUW47_RS11495	18186..18413	+	228	WP_007127136.1	hypothetical protein	-
BUW47_RS11500	18459..18716	+	258	WP_075667809.1	hypothetical protein	-
BUW47_RS11505	18706..19629	-	924	WP_075667810.1	zeta toxin family protein	Toxin
BUW47_RS11510	19632..19904	-	273	WP_007124950.1	epsilon-antitoxin	Antitoxin
BUW47_RS11895	20188..20485	-	298	Protein_22	hypothetical protein	-
BUW47_RS11520	20460..21623	-	1164	WP_021349372.1	replication initiator protein A	-
BUW47_RS11525	22176..22451	-	276	WP_007127113.1	hypothetical protein	-
BUW47_RS11530	22454..23503	-	1050	WP_075667812.1	ParM/StbA family protein	-
BUW47_RS11535	23878..24138	+	261	WP_040459087.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
BUW47_RS12055	24131..24502	+	372	WP_080967878.1	replication associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29166	29166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (23-213)

Antitoxin

• Bacterial epsilon antitoxin (4-90)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 35530.23 Da
• Isoelectric Point: 6.2682

>T71573 WP_075667810.1 NZ_CP019033:c19629-18706 [Limosilactobacillus fermentum]
MDELADYTDHQFETKLQIEIEQLTKNKQAQESPKAYLLGGQPGAGKSGLHQLIKAEDPNAITIDNDTFKWLHPKYKQLEQ
KYGKDVVKYVTPFSNQMTESLIDYLSDKKFDLIIEGTLRTVEVPMATVTKLQNRGYEASLYVMAVPRIESYLGTLARYED
QFSLSPRTARATTKEAHDVVVRQLPDNLDFLYKQRLFKEIRLYDRKGNKLYSSLENLNESPKKIITEILNRKLDNNTLLN
SIDSVINKMEINHHTTTPQYLDLVEKTTELKKEISGRVQEQLKEFAEKNPEVNPKEDPENKNDRPSY

Nucleotide

• Length: 924 bp

>T71573 NZ_CP019033:c19629-18706 [Limosilactobacillus fermentum]
ATGGACGAACTAGCTGACTATACCGATCATCAATTTGAAACGAAATTACAGATTGAAATCGAGCAATTAACCAAAAACAA
GCAAGCACAGGAAAGCCCCAAAGCTTATTTACTGGGCGGGCAACCGGGAGCGGGAAAGTCAGGCTTGCATCAACTCATCA
AAGCCGAAGATCCCAACGCTATTACGATTGATAACGATACTTTTAAGTGGCTACACCCAAAGTATAAACAACTAGAGCAA
AAATACGGGAAAGATGTTGTCAAATATGTAACCCCGTTTTCTAACCAAATGACTGAGTCTTTAATTGATTATCTGAGTGA
TAAAAAATTTGATTTAATTATCGAAGGGACGTTAAGAACAGTTGAAGTACCAATGGCCACTGTAACTAAGTTACAGAACA
GGGGTTACGAAGCGAGTTTATACGTTATGGCAGTTCCGAGAATTGAATCATATCTAGGGACTTTGGCACGCTATGAGGAT
CAATTTAGTTTATCACCTAGAACTGCTAGAGCGACTACCAAAGAAGCTCATGATGTCGTTGTCAGGCAGCTACCTGACAA
TTTAGATTTTCTGTATAAACAGAGGTTATTTAAAGAGATCAGACTATATGACCGAAAGGGTAACAAATTATATTCTAGCC
TAGAAAATTTGAACGAAAGCCCCAAAAAGATTATTACCGAGATTCTTAACAGAAAGCTAGATAATAATACCCTGCTTAAC
AGCATAGATAGTGTTATTAATAAAATGGAGATTAATCATCATACGACCACACCGCAGTACCTCGATTTAGTAGAAAAAAC
AACAGAACTAAAAAAAGAGATCAGCGGTAGGGTGCAGGAACAATTAAAAGAATTTGCTGAAAAGAATCCAGAAGTCAATC
CTAAAGAGGATCCAGAAAATAAAAATGATCGACCTAGTTATTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10340.91 Da
• Isoelectric Point: 5.5093

>AT71573 WP_007124950.1 NZ_CP019033:c19904-19632 [Limosilactobacillus fermentum]
MKLDYQQTYKKEILTEFASSIYAKVVNLVVDQELNIHDESHFLVKLMHQLGDAKLVIMDAHSLGELETIQAYWQAMNNFI
DSLPTKSKVA

Nucleotide

• Length: 273 bp

>AT71573 NZ_CP019033:c19904-19632 [Limosilactobacillus fermentum]
ATGAAATTAGACTATCAACAAACTTACAAAAAGGAAATTCTAACCGAATTTGCCAGCAGCATTTATGCTAAAGTAGTCAA
TTTGGTCGTTGACCAAGAATTAAATATTCACGATGAAAGTCATTTTCTGGTTAAACTGATGCACCAGTTAGGAGATGCTA
AGTTAGTAATTATGGACGCGCATTCCTTGGGAGAGTTAGAGACAATTCAAGCTTACTGGCAAGCAATGAATAACTTTATT
GATAGTCTGCCAACGAAAAGTAAAGTTGCTTAG

Structures

Antitoxin

• AlphaFold DB: V5E259