Detailed information of TA system TA071572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 675..1276
• Replicon: plasmid pSNU175-4
• Accession: NZ_CP019033
• Organism: Limosilactobacillus fermentum strain SNUV175

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: U6FEM0
• Locus tag: BUW47_RS11410
• Protein ID: WP_011241744.1
• Coordinates: 932..1276 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BUW47_RS11405
• Protein ID: WP_049160264.1
• Coordinates: 675..938 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUW47_RS11400	355..579	-	225	Protein_1	hypothetical protein	-
BUW47_RS11405	675..938	+	264	WP_049160264.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BUW47_RS11410	932..1276	+	345	WP_011241744.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BUW47_RS11415	1565..1786	-	222	WP_075667799.1	hypothetical protein	-
BUW47_RS11420	1814..2080	+	267	WP_187337535.1	hypothetical protein	-
BUW47_RS11425	2269..2613	-	345	WP_075667801.1	hypothetical protein	-
BUW47_RS11430	2606..2980	-	375	WP_075667802.1	hypothetical protein	-
BUW47_RS11435	3017..4318	-	1302	WP_075667803.1	hypothetical protein	-
BUW47_RS11440	4332..4775	-	444	WP_075667804.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29166	29166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-101)

Antitoxin

• SpoVT-AbrB domain (12-55)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13010.79 Da
• Isoelectric Point: 5.5556

>T71572 WP_011241744.1 NZ_CP019033:932-1276 [Limosilactobacillus fermentum]
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVADEAIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T71572 NZ_CP019033:932-1276 [Limosilactobacillus fermentum]
ATGGTAAGTCAGGGCGATATATTCTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAGGCGGCCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGCCAAACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCGAGCGGAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTGTTAGATCAAACAATAGCTTTAGATTTGCGG
GCAAGACATGTTGCTGACGAAGCTATTGTCGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCACTCTGTACAAATT
ATTGTTTAGTATTGATGACAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10169.48 Da
• Isoelectric Point: 5.7117

>AT71572 WP_049160264.1 NZ_CP019033:675-938 [Limosilactobacillus fermentum]
MELIKEQTRLAKWGNSKAARIPSQIIRQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT71572 NZ_CP019033:675-938 [Limosilactobacillus fermentum]
ATGGAACTTATTAAAGAACAAACACGCCTGGCAAAGTGGGGCAACTCGAAAGCTGCTAGAATTCCTAGCCAGATTATTAG
ACAATTGAAATTAGATGATAACCAAGATATGACAATAACAATTGAAAATGGATCGATTGTTTTAACACCAATAAAAAAGA
ATCCGACTAATATTCACGAACTATTTAAGGACTGGCAAGATGACGGGAAACGTGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: U6FEM0