Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 96005..96606 Replicon plasmid pEC881_1
Accession NZ_CP019028
Organism Escherichia coli strain Ecol_881

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BE930_RS01895 Protein ID WP_001216034.1
Coordinates 96005..96385 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BE930_RS01900 Protein ID WP_001190712.1
Coordinates 96385..96606 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE930_RS01860 91056..91988 - 933 WP_000081352.1 homocysteine S-methyltransferase -
BE930_RS01865 91975..93378 - 1404 WP_001373486.1 S-methylmethionine permease -
BE930_RS01870 93622..94602 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
BE930_RS01875 94830..95147 + 318 WP_001513659.1 hypothetical protein -
BE930_RS01880 95097..95510 - 414 Protein_117 DDE-type integrase/transposase/recombinase -
BE930_RS01885 95434..95793 - 360 WP_001513660.1 hypothetical protein -
BE930_RS01890 95821..96000 - 180 WP_001513661.1 hypothetical protein -
BE930_RS01895 96005..96385 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BE930_RS01900 96385..96606 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BE930_RS01905 96789..98345 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
BE930_RS01910 98342..99625 + 1284 WP_001553855.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B) - 1..147346 147346


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T71535 WP_001216034.1 NZ_CP019028:c96385-96005 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T71535 NZ_CP019028:c96385-96005 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT71535 WP_001190712.1 NZ_CP019028:c96606-96385 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT71535 NZ_CP019028:c96606-96385 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References