71534

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 81354..81913 Replicon plasmid pEC881_1
Accession NZ_CP019028
Organism Escherichia coli strain Ecol_881

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag BE930_RS01780 Protein ID WP_000421272.1
Coordinates 81638..81913 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag BE930_RS01775 Protein ID WP_001387467.1
Coordinates 81354..81638 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE930_RS01745 76817..77257 - 441 Protein_95 zinc-binding dehydrogenase -
BE930_RS01755 77658..78548 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
BE930_RS01760 78573..78953 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
BE930_RS01765 78986..79951 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
BE930_RS01770 79997..80749 - 753 WP_000562172.1 IclR family transcriptional regulator -
BE930_RS01775 81354..81638 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
BE930_RS01780 81638..81913 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE930_RS01785 82029..82726 - 698 Protein_102 IS1-like element IS1A family transposase -
BE930_RS01795 82796..83089 + 294 WP_001105060.1 hypothetical protein -
BE930_RS29235 83124..83252 - 129 Protein_104 IS481 family transposase -
BE930_RS01800 83285..84454 - 1170 WP_001072355.1 hypothetical protein -
BE930_RS01810 84947..85254 + 308 Protein_106 type II toxin-antitoxin system ParD family antitoxin -
BE930_RS29240 85597..85779 + 183 WP_042065278.1 hypothetical protein -
BE930_RS01825 85900..86640 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B) - 1..147346 147346


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T71534 WP_000421272.1 NZ_CP019028:81638-81913 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T71534 NZ_CP019028:81638-81913 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT71534 WP_001387467.1 NZ_CP019028:81354-81638 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT71534 NZ_CP019028:81354-81638 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References