Detailed information of TA system TA071528

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3700908..3701609
• Replicon: chromosome
• Accession: NZ_CP019020
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: BE937_RS21895
• Protein ID: WP_001531265.1
• Coordinates: 3700908..3701243 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BE937_RS21900
• Protein ID: WP_032143152.1
• Coordinates: 3701274..3701609 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS21865	3697847..3698018	-	172	Protein_3530	integrase	-
BE937_RS21875	3698429..3699226	-	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
BE937_RS21880	3699340..3699615	-	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BE937_RS21885	3699619..3699867	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
BE937_RS21890	3699944..3700777	-	834	WP_001531266.1	DUF4942 domain-containing protein	-
BE937_RS21895	3700908..3701243	-	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
BE937_RS21900	3701274..3701609	-	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE937_RS21905	3701609..3702082	-	474	WP_001531261.1	DNA repair protein RadC	-
BE937_RS21910	3702112..3702930	-	819	WP_001531260.1	DUF945 domain-containing protein	-
BE937_RS21915	3703166..3704119	-	954	WP_001531259.1	hypothetical protein	-
BE937_RS21920	3704712..3705326	+	615	WP_001531257.1	inovirus Gp2 family protein	-
BE937_RS21925	3705444..3705662	+	219	WP_001531256.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T71528 WP_001531265.1 NZ_CP019020:c3701243-3700908 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T71528 NZ_CP019020:c3701243-3700908 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT71528 WP_032143152.1 NZ_CP019020:c3701609-3701274 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT71528 NZ_CP019020:c3701609-3701274 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3