Detailed information of TA system TA071508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 155386..155911
• Replicon: plasmid pEC244_2
• Accession: NZ_CP019018
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: BE937_RS01690
• Protein ID: WP_001159868.1
• Coordinates: 155606..155911 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: BE937_RS01685
• Protein ID: WP_000813634.1
• Coordinates: 155386..155604 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS01650	150463..150810	+	348	WP_000142449.1	hypothetical protein	-
BE937_RS01655	150830..151420	-	591	WP_000194553.1	hypothetical protein	-
BE937_RS01660	151420..151677	-	258	WP_000371889.1	hypothetical protein	-
BE937_RS01665	152005..153108	+	1104	WP_001127573.1	nucleotide-binding protein	-
BE937_RS01670	153138..154268	+	1131	WP_000542418.1	DUF4238 domain-containing protein	-
BE937_RS01675	154268..154558	+	291	WP_001229374.1	hypothetical protein	-
BE937_RS01685	155386..155604	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BE937_RS01690	155606..155911	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
BE937_RS01695	155912..156718	+	807	WP_001593069.1	site-specific integrase	-
BE937_RS01700	156898..157542	+	645	WP_001144036.1	ParA family protein	-
BE937_RS01705	157629..157937	+	309	WP_000030198.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158911	158911
	-	flank	IS/Tn	-	-	158150..158527	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T71508 WP_001159868.1 NZ_CP019018:155606-155911 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T71508 NZ_CP019018:155606-155911 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT71508 WP_000813634.1 NZ_CP019018:155386-155604 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT71508 NZ_CP019018:155386-155604 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2