Detailed information of TA system TA071497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 12468..13111
• Replicon: plasmid pEC244_KPC
• Accession: NZ_CP019017
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BE937_RS00370
• Protein ID: WP_001044770.1
• Coordinates: 12695..13111 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BE937_RS00365
• Protein ID: WP_001261282.1
• Coordinates: 12468..12698 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS00345	9989..10765	-	777	WP_000015958.1	site-specific integrase	-
BE937_RS00350	10762..11505	-	744	WP_000129823.1	hypothetical protein	-
BE937_RS00355	11556..11906	-	351	WP_000493378.1	hypothetical protein	-
BE937_RS28440	12050..12511	-	462	WP_072202616.1	hypothetical protein	-
BE937_RS00365	12468..12698	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE937_RS00370	12695..13111	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE937_RS00375	13185..13895	+	711	WP_004152334.1	ATP-binding protein	-
BE937_RS00380	13929..14626	+	698	Protein_18	IS1 family transposase	-
BE937_RS00385	14642..14752	+	111	Protein_19	mercuric transport protein periplasmic component	-
BE937_RS00390	14788..15210	+	423	WP_001340589.1	organomercurial transporter MerC	-
BE937_RS00395	15262..16956	+	1695	WP_000105636.1	mercury(II) reductase	-
BE937_RS00400	16974..17336	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
BE937_RS00405	17333..17569	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(6')-Ib / blaOXA-9 / blaTEM-1A / blaKPC-2 / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2	-	1..73464	73464
	-	inside	IScluster/Tn	aac(6')-Ib	-	4150..14626	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T71497 WP_001044770.1 NZ_CP019017:12695-13111 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71497 NZ_CP019017:12695-13111 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT71497 WP_001261282.1 NZ_CP019017:12468-12698 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT71497 NZ_CP019017:12468-12698 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3