Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 4732..5254 Replicon plasmid pEC244_4
Accession NZ_CP019016
Organism Escherichia coli strain Ecol_244

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag BE937_RS00060 Protein ID WP_000220561.1
Coordinates 4732..5013 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BE937_RS00065 Protein ID WP_000121743.1
Coordinates 5003..5254 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE937_RS00005 1..348 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
BE937_RS00010 345..593 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
BE937_RS00015 899..1249 - 351 WP_000854261.1 hypothetical protein -
BE937_RS00020 1341..1640 - 300 WP_000650311.1 hypothetical protein -
BE937_RS00030 2094..2321 - 228 WP_000801440.1 hypothetical protein -
BE937_RS00035 2308..2559 - 252 WP_001230707.1 hypothetical protein -
BE937_RS00040 2556..3098 - 543 WP_001083906.1 hypothetical protein -
BE937_RS00045 3203..3718 - 516 WP_001025397.1 J domain-containing protein -
BE937_RS00055 4358..4708 - 351 WP_000312755.1 hypothetical protein -
BE937_RS00060 4732..5013 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE937_RS00065 5003..5254 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BE937_RS00070 6620..7627 + 1008 WP_000243325.1 replication initiation protein -
BE937_RS00075 7693..8097 + 405 WP_000654312.1 hypothetical protein -
BE937_RS00080 8233..8760 + 528 WP_078234943.1 DUF2726 domain-containing protein -
BE937_RS00095 9332..9583 - 252 WP_000905709.1 hypothetical protein -
BE937_RS00100 9620..9922 - 303 WP_001062963.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37756 37756


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T71496 WP_000220561.1 NZ_CP019016:c5013-4732 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T71496 NZ_CP019016:c5013-4732 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTTGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT71496 WP_000121743.1 NZ_CP019016:c5254-5003 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT71496 NZ_CP019016:c5254-5003 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References