Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1..593 Replicon plasmid pEC244_4
Accession NZ_CP019016
Organism Escherichia coli strain Ecol_244

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag BE937_RS00010 Protein ID WP_000520549.1
Coordinates 345..593 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag BE937_RS00005 Protein ID WP_000681613.1
Coordinates 1..348 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE937_RS00005 1..348 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BE937_RS00010 345..593 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
BE937_RS00015 899..1249 - 351 WP_000854261.1 hypothetical protein -
BE937_RS00020 1341..1640 - 300 WP_000650311.1 hypothetical protein -
BE937_RS00030 2094..2321 - 228 WP_000801440.1 hypothetical protein -
BE937_RS00035 2308..2559 - 252 WP_001230707.1 hypothetical protein -
BE937_RS00040 2556..3098 - 543 WP_001083906.1 hypothetical protein -
BE937_RS00045 3203..3718 - 516 WP_001025397.1 J domain-containing protein -
BE937_RS00055 4358..4708 - 351 WP_000312755.1 hypothetical protein -
BE937_RS00060 4732..5013 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin -
BE937_RS00065 5003..5254 - 252 WP_000121743.1 plasmid stabilization protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37756 37756


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T71494 WP_000520549.1 NZ_CP019016:c593-345 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T71494 NZ_CP019016:c593-345 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT71494 WP_000681613.1 NZ_CP019016:c348-1 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT71494 NZ_CP019016:c348-1 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGACTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References