Detailed information of TA system TA071478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2185345..2186145
• Replicon: chromosome
• Accession: NZ_CP019015
• Organism: Escherichia coli strain Ecol_AZ162

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: BE965_RS12665
• Protein ID: WP_000342452.1
• Coordinates: 2185618..2186145 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: BE965_RS12660
• Protein ID: WP_001277107.1
• Coordinates: 2185345..2185611 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE965_RS12640	2181004..2181672	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
BE965_RS12645	2181665..2182723	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
BE965_RS12650	2182968..2183822	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
BE965_RS12655	2184093..2185196	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
BE965_RS12660	2185345..2185611	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
BE965_RS12665	2185618..2186145	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
BE965_RS12670	2186142..2186525	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
BE965_RS12675	2186948..2188057	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
BE965_RS12680	2188105..2189031	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BE965_RS12685	2189028..2190305	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
BE965_RS12690	2190302..2191069	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T71478 WP_000342452.1 NZ_CP019015:2185618-2186145 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T71478 NZ_CP019015:2185618-2186145 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT71478 WP_001277107.1 NZ_CP019015:2185345-2185611 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT71478 NZ_CP019015:2185345-2185611 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57