Detailed information of TA system TA071475

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1359874..1360709
• Replicon: chromosome
• Accession: NZ_CP019015
• Organism: Escherichia coli strain Ecol_AZ162

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5F0P695
• Locus tag: BE965_RS08655
• Protein ID: WP_022645116.1
• Coordinates: 1360332..1360709 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: BE965_RS08650
• Protein ID: WP_001295723.1
• Coordinates: 1359874..1360242 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE965_RS08610	1355538..1356218	+	681	WP_001282921.1	WYL domain-containing protein	-
BE965_RS08615	1356366..1357043	+	678	WP_001097302.1	hypothetical protein	-
BE965_RS08620	1357049..1357282	+	234	WP_022645118.1	DUF905 family protein	-
BE965_RS08625	1357372..1358190	+	819	WP_001175165.1	DUF945 domain-containing protein	-
BE965_RS08630	1358456..1358935	+	480	WP_001564060.1	antirestriction protein	-
BE965_RS08635	1358951..1359427	+	477	WP_022645117.1	RadC family protein	-
BE965_RS08640	1359490..1359711	+	222	WP_000692345.1	DUF987 domain-containing protein	-
BE965_RS08650	1359874..1360242	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE965_RS08655	1360332..1360709	+	378	WP_022645116.1	TA system toxin CbtA family protein	Toxin
BE965_RS28710	1360706..1360855	+	150	Protein_1295	hypothetical protein	-
BE965_RS28230	1360934..1361128	+	195	WP_024181941.1	DUF957 domain-containing protein	-
BE965_RS08665	1361213..1362055	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
BE965_RS08675	1362804..1364342	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1326374..1387746	61372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14021.99 Da
• Isoelectric Point: 7.8045

>T71475 WP_022645116.1 NZ_CP019015:1360332-1360709 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T71475 NZ_CP019015:1360332-1360709 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT71475 WP_001295723.1 NZ_CP019015:1359874-1360242 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71475 NZ_CP019015:1359874-1360242 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5F0P695

Antitoxin

• AlphaFold DB: S1NM52