Detailed information of TA system TA071473

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1103447..1104282
• Replicon: chromosome
• Accession: NZ_CP019015
• Organism: Escherichia coli strain Ecol_AZ162

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: BE965_RS07185
• Protein ID: WP_000854759.1
• Coordinates: 1103447..1103824 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: BE965_RS07190
• Protein ID: WP_001295723.1
• Coordinates: 1103914..1104282 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE965_RS07160	1099558..1101198	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
BE965_RS28160	1101971..1102147	-	177	Protein_1030	helix-turn-helix domain-containing protein	-
BE965_RS28695	1102511..1102669	-	159	WP_001467148.1	hypothetical protein	-
BE965_RS07175	1102769..1102945	-	177	WP_000839286.1	DUF957 domain-containing protein	-
BE965_RS07180	1102962..1103450	-	489	WP_000761690.1	hypothetical protein	-
BE965_RS07185	1103447..1103824	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
BE965_RS07190	1103914..1104282	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE965_RS07200	1104445..1104666	-	222	WP_000692345.1	DUF987 domain-containing protein	-
BE965_RS07205	1104729..1105205	-	477	WP_001186775.1	RadC family protein	-
BE965_RS07210	1105221..1105694	-	474	WP_001350782.1	antirestriction protein	-
BE965_RS07220	1106036..1106854	-	819	WP_022645130.1	DUF945 domain-containing protein	-
BE965_RS07230	1107009..1107167	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T71473 WP_000854759.1 NZ_CP019015:c1103824-1103447 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T71473 NZ_CP019015:c1103824-1103447 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT71473 WP_001295723.1 NZ_CP019015:c1104282-1103914 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71473 NZ_CP019015:c1104282-1103914 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52