Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 912655..913175 Replicon chromosome
Accession NZ_CP019015
Organism Escherichia coli strain Ecol_AZ162

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag BE965_RS06205 Protein ID WP_001160964.1
Coordinates 912655..912939 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag BE965_RS06210 Protein ID WP_000125566.1
Coordinates 912942..913175 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE965_RS06180 908300..909586 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
BE965_RS06185 909586..910575 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BE965_RS06190 910572..911393 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BE965_RS06195 911396..911773 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BE965_RS06200 911780..912628 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BE965_RS06205 912655..912939 - 285 WP_001160964.1 CcdB family protein Toxin
BE965_RS06210 912942..913175 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BE965_RS06215 913261..913740 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
BE965_RS06220 913932..915794 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
BE965_RS06225 915787..916317 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BE965_RS06230 916425..917756 - 1332 WP_022645025.1 MFS transporter -
BE965_RS06235 917815..918102 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T71470 WP_001160964.1 NZ_CP019015:c912939-912655 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T71470 NZ_CP019015:c912939-912655 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT71470 WP_000125566.1 NZ_CP019015:c913175-912942 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT71470 NZ_CP019015:c913175-912942 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References