Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 68099..68658 Replicon plasmid pECAZ162_KPC
Accession NZ_CP019014
Organism Escherichia coli strain Ecol_AZ162

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag BE965_RS01100 Protein ID WP_000421272.1
Coordinates 68383..68658 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag BE965_RS01095 Protein ID WP_001387467.1
Coordinates 68099..68383 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE965_RS01065 63562..64002 - 441 Protein_71 zinc-binding dehydrogenase -
BE965_RS01070 63976..64170 + 195 WP_000796505.1 hypothetical protein -
BE965_RS01075 64403..65293 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
BE965_RS01080 65318..65698 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
BE965_RS01085 65731..66696 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
BE965_RS01090 66742..67494 - 753 WP_000562172.1 IclR family transcriptional regulator -
BE965_RS01095 68099..68383 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
BE965_RS01100 68383..68658 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE965_RS01105 68764..69057 + 294 WP_001105060.1 hypothetical protein -
BE965_RS28040 69092..69220 - 129 Protein_80 IS481 family transposase -
BE965_RS01110 69253..70422 - 1170 WP_001072355.1 hypothetical protein -
BE965_RS01120 70915..71222 + 308 Protein_82 type II toxin-antitoxin system ParD family antitoxin -
BE965_RS01135 71511..72208 + 698 Protein_83 IS1 family transposase -
BE965_RS01145 72462..73484 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3'')-Ib / aph(6)-Id / blaKPC-3 / tet(A) / sul1 / qacE / aadA5 / sul2 - 1..142829 142829
- flank IS/Tn - - 71705..72208 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T71459 WP_000421272.1 NZ_CP019014:68383-68658 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T71459 NZ_CP019014:68383-68658 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT71459 WP_001387467.1 NZ_CP019014:68099-68383 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT71459 NZ_CP019014:68099-68383 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References