Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 4710209..4711797 Replicon chromosome
Accession NZ_CP019012
Organism Escherichia coli strain Ecol_AZ161

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag BE946_RS26020 Protein ID WP_001125411.1
Coordinates 4710475..4711797 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag BE946_RS26015 Protein ID WP_001296076.1
Coordinates 4710209..4710475 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE946_RS25995 4706577..4708028 + 1452 WP_000854638.1 tagaturonate reductase -
BE946_RS26005 4708225..4709139 + 915 WP_001296077.1 bestrophin family protein -
BE946_RS26010 4709143..4709901 - 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
BE946_RS26015 4710209..4710475 + 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
BE946_RS26020 4710475..4711797 + 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
BE946_RS26030 4712150..4712860 + 711 WP_001195176.1 fimbria/pilus periplasmic chaperone -
BE946_RS26035 4712902..4715553 + 2652 WP_001531687.1 fimbrial biogenesis outer membrane usher protein -
BE946_RS26040 4715567..4716097 + 531 WP_000876789.1 type 1 fimbrial protein -
BE946_RS26045 4716110..4716613 + 504 WP_000825466.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T71458 WP_001125411.1 NZ_CP019012:4710475-4711797 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T71458 NZ_CP019012:4710475-4711797 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT71458 WP_001296076.1 NZ_CP019012:4710209-4710475 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT71458 NZ_CP019012:4710209-4710475 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References