Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4170739..4171570 Replicon chromosome
Accession NZ_CP019012
Organism Escherichia coli strain Ecol_AZ161

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag BE946_RS22970 Protein ID WP_000854815.1
Coordinates 4170739..4171113 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag BE946_RS22975 Protein ID WP_001280918.1
Coordinates 4171202..4171570 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE946_RS22935 4166143..4167363 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
BE946_RS22940 4167420..4167893 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
BE946_RS22945 4168091..4169149 + 1059 WP_001200889.1 FUSC family protein -
BE946_RS22950 4169321..4169650 + 330 WP_000450409.1 DUF496 family protein -
BE946_RS22955 4169751..4169933 - 183 WP_001296208.1 hypothetical protein -
BE946_RS22960 4169987..4170133 + 147 Protein_3916 transposase domain-containing protein -
BE946_RS27465 4170422..4170535 - 114 WP_001161660.1 DUF957 domain-containing protein -
BE946_RS22965 4170548..4170742 - 195 WP_000988600.1 hypothetical protein -
BE946_RS22970 4170739..4171113 - 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
BE946_RS22975 4171202..4171570 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BE946_RS22980 4171586..4172230 - 645 WP_000086752.1 hypothetical protein -
BE946_RS22985 4172249..4172470 - 222 WP_000692345.1 DUF987 domain-containing protein -
BE946_RS22990 4172533..4173009 - 477 WP_001186200.1 RadC family protein -
BE946_RS22995 4173025..4173498 - 474 WP_001542276.1 antirestriction protein -
BE946_RS23000 4173592..4173837 - 246 WP_001164966.1 hypothetical protein -
BE946_RS23005 4173837..4174655 - 819 WP_001542275.1 DUF945 domain-containing protein -
BE946_RS23015 4174876..4175286 - 411 WP_000846703.1 hypothetical protein -
BE946_RS23020 4175302..4175652 - 351 Protein_3928 hypothetical protein -
BE946_RS23025 4175735..4176481 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T71449 WP_000854815.1 NZ_CP019012:c4171113-4170739 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T71449 NZ_CP019012:c4171113-4170739 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT71449 WP_001280918.1 NZ_CP019012:c4171570-4171202 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT71449 NZ_CP019012:c4171570-4171202 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References