Detailed information of TA system TA071433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 48012..48571
• Replicon: plasmid pECAZ161_1
• Accession: NZ_CP019011
• Organism: Escherichia coli strain Ecol_AZ161

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: BE946_RS01490
• Protein ID: WP_000421259.1
• Coordinates: 48296..48571 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: BE946_RS01485
• Protein ID: WP_001178087.1
• Coordinates: 48012..48296 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE946_RS01450	43201..43821	-	621	WP_000176497.1	DUF1449 family protein	-
BE946_RS01460	44088..44750	-	663	WP_000154141.1	hypothetical protein	-
BE946_RS01465	44891..45532	-	642	WP_000335849.1	transcription termination factor NusG	-
BE946_RS01475	46235..47113	+	879	WP_000729439.1	incFII family plasmid replication initiator RepA	-
BE946_RS01485	48012..48296	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
BE946_RS01490	48296..48571	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE946_RS01495	48677..48952	+	276	WP_001401588.1	hypothetical protein	-
BE946_RS27805	49143..49409	-	267	WP_000627832.1	hypothetical protein	-
BE946_RS27810	49409..49645	-	237	WP_032151844.1	hypothetical protein	-
BE946_RS01515	49655..50257	-	603	WP_000521606.1	hypothetical protein	-
BE946_RS01520	50433..51485	-	1053	WP_000655519.1	hypothetical protein	-
BE946_RS01525	51698..52288	-	591	WP_001401589.1	hypothetical protein	-
BE946_RS01530	52288..52542	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74891	74891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T71433 WP_000421259.1 NZ_CP019011:48296-48571 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T71433 NZ_CP019011:48296-48571 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAAGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT71433 WP_001178087.1 NZ_CP019011:48012-48296 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT71433 NZ_CP019011:48012-48296 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625