Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 86822..87347 Replicon plasmid pECAZ161_3
Accession NZ_CP019009
Organism Escherichia coli strain Ecol_AZ161

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BE946_RS00595 Protein ID WP_001159868.1
Coordinates 86822..87127 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BE946_RS00600 Protein ID WP_000813634.1
Coordinates 87129..87347 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE946_RS00580 82732..83898 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BE946_RS00585 84486..85241 - 756 WP_000852146.1 replication initiation protein RepE -
BE946_RS00590 86015..86821 - 807 WP_000016982.1 site-specific integrase -
BE946_RS00595 86822..87127 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BE946_RS00600 87129..87347 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE946_RS00605 88055..89050 + 996 WP_000246636.1 hypothetical protein -
BE946_RS00610 89054..89986 + 933 WP_000991832.1 hypothetical protein -
BE946_RS00615 90123..90820 - 698 Protein_107 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 - 1..130905 130905
- flank IS/Tn - - 90123..90626 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T71431 WP_001159868.1 NZ_CP019009:c87127-86822 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71431 NZ_CP019009:c87127-86822 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT71431 WP_000813634.1 NZ_CP019009:c87347-87129 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71431 NZ_CP019009:c87347-87129 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References