Detailed information of TA system TA071421

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4439009..4439710
• Replicon: chromosome
• Accession: NZ_CP019008
• Organism: Escherichia coli strain Ecol_AZ159

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: BE939_RS23880
• Protein ID: WP_001531265.1
• Coordinates: 4439009..4439344 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BE939_RS23885
• Protein ID: WP_032143152.1
• Coordinates: 4439375..4439710 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE939_RS23850	4435948..4436119	-	172	Protein_4193	integrase	-
BE939_RS23860	4436530..4437327	-	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
BE939_RS23865	4437441..4437716	-	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BE939_RS23870	4437720..4437968	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
BE939_RS23875	4438045..4438878	-	834	WP_001531266.1	DUF4942 domain-containing protein	-
BE939_RS23880	4439009..4439344	-	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
BE939_RS23885	4439375..4439710	-	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE939_RS23890	4439710..4440183	-	474	WP_001531261.1	DNA repair protein RadC	-
BE939_RS23895	4440213..4441031	-	819	WP_001531260.1	DUF945 domain-containing protein	-
BE939_RS23900	4441267..4442220	-	954	WP_001531259.1	hypothetical protein	-
BE939_RS23905	4442813..4443427	+	615	WP_001531257.1	inovirus Gp2 family protein	-
BE939_RS23910	4443545..4443763	+	219	WP_001531256.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T71421 WP_001531265.1 NZ_CP019008:c4439344-4439009 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T71421 NZ_CP019008:c4439344-4439009 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT71421 WP_032143152.1 NZ_CP019008:c4439710-4439375 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT71421 NZ_CP019008:c4439710-4439375 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3