Detailed information of TA system TA071399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 76366..76913
• Replicon: plasmid pECAZ159_1
• Accession: NZ_CP019007
• Organism: Escherichia coli strain Ecol_AZ159

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BE939_RS00850
• Protein ID: WP_000969990.1
• Coordinates: 76632..76913 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: BE939_RS00845
• Protein ID: WP_000079941.1
• Coordinates: 76366..76635 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE939_RS00830	73137..74411	-	1275	WP_000489607.1	HlyD family secretion protein	-
BE939_RS00835	74608..75844	-	1237	Protein_87	IS3 family transposase	-
BE939_RS00845	76366..76635	+	270	WP_000079941.1	hypothetical protein	Antitoxin
BE939_RS00850	76632..76913	+	282	WP_000969990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE939_RS00855	76959..77807	+	849	WP_001058005.1	3'-5' exonuclease	-
BE939_RS00860	77924..78406	+	483	WP_001311056.1	hypothetical protein	-
BE939_RS00870	78708..79055	+	348	WP_000142437.1	hypothetical protein	-
BE939_RS00880	79614..80648	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB	1..128248	128248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10930.61 Da
• Isoelectric Point: 8.4615

>T71399 WP_000969990.1 NZ_CP019007:76632-76913 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T71399 NZ_CP019007:76632-76913 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT71399 WP_000079941.1 NZ_CP019007:76366-76635 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT71399 NZ_CP019007:76366-76635 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5