Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 50386..50933 Replicon plasmid pECAZ159_1
Accession NZ_CP019007
Organism Escherichia coli strain Ecol_AZ159

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag BE939_RS00700 Protein ID WP_000969996.1
Coordinates 50652..50933 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID W0FTF6
Locus tag BE939_RS00695 Protein ID WP_000079942.1
Coordinates 50386..50655 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE939_RS00645 46039..46251 + 213 WP_012311405.1 hypothetical protein -
BE939_RS00650 46494..46955 + 462 WP_001233871.1 thermonuclease family protein -
BE939_RS00655 47001..47210 + 210 WP_001333231.1 hemolysin expression modulator Hha -
BE939_RS00660 47248..47821 + 574 Protein_59 DUF2726 domain-containing protein -
BE939_RS00665 48060..48320 + 261 WP_000083818.1 replication regulatory protein RepA -
BE939_RS00675 48546..48620 + 75 WP_001365705.1 RepA leader peptide Tap -
BE939_RS00680 48613..49470 + 858 WP_031942351.1 incFII family plasmid replication initiator RepA -
BE939_RS00695 50386..50655 + 270 WP_000079942.1 hypothetical protein Antitoxin
BE939_RS00700 50652..50933 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE939_RS00705 50979..51191 + 213 Protein_65 3'-5' exonuclease -
BE939_RS00710 51450..52379 - 930 WP_000102288.1 hypothetical protein -
BE939_RS00715 52918..54495 - 1578 WP_001323889.1 PAS domain-containing methyl-accepting chemotaxis protein -
BE939_RS00720 54805..55365 + 561 WP_001161490.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroN / iroE / iroD / iroC / iroB 1..128248 128248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T71398 WP_000969996.1 NZ_CP019007:50652-50933 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T71398 NZ_CP019007:50652-50933 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10282.61 Da        Isoelectric Point: 7.2057

>AT71398 WP_000079942.1 NZ_CP019007:50386-50655 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT71398 NZ_CP019007:50386-50655 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCCGGACGTCAGCAACTTGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A140JYS5

References