Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3935438..3936063 Replicon chromosome
Accession NZ_CP019005
Organism Escherichia coli strain Ecol_AZ155

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag BE948_RS22870 Protein ID WP_000911329.1
Coordinates 3935438..3935836 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag BE948_RS22875 Protein ID WP_000450524.1
Coordinates 3935836..3936063 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE948_RS22850 3931316..3931516 + 201 WP_000383836.1 YpfN family protein -
BE948_RS22855 3931626..3932324 - 699 WP_000679812.1 esterase -
BE948_RS22860 3932398..3934413 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
BE948_RS22865 3934428..3935291 - 864 WP_001267507.1 neutral zinc metallopeptidase -
BE948_RS22870 3935438..3935836 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
BE948_RS22875 3935836..3936063 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
BE948_RS22880 3936219..3936932 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
BE948_RS22885 3937145..3938179 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
BE948_RS22890 3938196..3939074 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
BE948_RS22895 3939220..3939792 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
BE948_RS22900 3939792..3940262 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T71389 WP_000911329.1 NZ_CP019005:c3935836-3935438 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T71389 NZ_CP019005:c3935836-3935438 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT71389 WP_000450524.1 NZ_CP019005:c3936063-3935836 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT71389 NZ_CP019005:c3936063-3935836 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References