Detailed information of TA system TA071372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1301648..1302224
• Replicon: chromosome
• Accession: NZ_CP019005
• Organism: Escherichia coli strain Ecol_AZ155

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: BE948_RS09310
• Protein ID: WP_001297643.1
• Coordinates: 1301648..1301935 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: BE948_RS09315
• Protein ID: WP_000063156.1
• Coordinates: 1301922..1302224 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE948_RS09290	1297901..1299181	-	1281	WP_001037380.1	DUF445 domain-containing protein	-
BE948_RS09295	1299222..1300454	-	1233	WP_001380906.1	multidrug efflux MFS transporter MdtM	-
BE948_RS09300	1300600..1300695	-	96	Protein_1200	dienelactone hydrolase family protein	-
BE948_RS09305	1300752..1301449	+	698	Protein_1201	IS1 family transposase	-
BE948_RS09310	1301648..1301935	+	288	WP_001297643.1	BrnT family toxin	Toxin
BE948_RS09315	1301922..1302224	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
BE948_RS09320	1302258..1303214	-	957	WP_001297640.1	GTPase	-
BE948_RS09325	1303225..1303428	-	204	WP_000467859.1	YbdD/YjiX family protein	-
BE948_RS09330	1303478..1305628	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	cheD	1300946..1320740	19794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T71372 WP_001297643.1 NZ_CP019005:1301648-1301935 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T71372 NZ_CP019005:1301648-1301935 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT71372 WP_000063156.1 NZ_CP019005:1301922-1302224 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT71372 NZ_CP019005:1301922-1302224 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0