71356

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4103461..4103981 Replicon chromosome
Accession NZ_CP019000
Organism Escherichia coli strain Ecol_AZ153

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag BE940_RS23140 Protein ID WP_001160964.1
Coordinates 4103697..4103981 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag BE940_RS23135 Protein ID WP_000125566.1
Coordinates 4103461..4103694 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE940_RS23110 4098534..4098821 + 288 WP_000203744.1 ferredoxin-like protein FixX -
BE940_RS23115 4098880..4100211 + 1332 WP_001183209.1 MFS transporter -
BE940_RS23120 4100319..4100849 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BE940_RS23125 4100842..4102704 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
BE940_RS23130 4102896..4103375 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
BE940_RS23135 4103461..4103694 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BE940_RS23140 4103697..4103981 + 285 WP_001160964.1 CcdB family protein Toxin
BE940_RS23145 4104008..4104856 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BE940_RS23150 4104863..4105240 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BE940_RS23155 4105243..4106064 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BE940_RS23160 4106061..4107050 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BE940_RS23165 4107050..4108336 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T71356 WP_001160964.1 NZ_CP019000:4103697-4103981 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T71356 NZ_CP019000:4103697-4103981 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT71356 WP_000125566.1 NZ_CP019000:4103461-4103694 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT71356 NZ_CP019000:4103461-4103694 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References