Detailed information of TA system TA071350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2873838..2874638
• Replicon: chromosome
• Accession: NZ_CP019000
• Organism: Escherichia coli strain Ecol_AZ153

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: BE940_RS16980
• Protein ID: WP_000342452.1
• Coordinates: 2873838..2874365 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: BE940_RS16985
• Protein ID: WP_001277107.1
• Coordinates: 2874372..2874638 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE940_RS16955	2868914..2869681	-	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
BE940_RS16960	2869678..2870955	-	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
BE940_RS16965	2870952..2871878	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BE940_RS16970	2871926..2873035	-	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
BE940_RS16975	2873458..2873841	+	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
BE940_RS16980	2873838..2874365	-	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
BE940_RS16985	2874372..2874638	-	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
BE940_RS16990	2874787..2875890	-	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
BE940_RS16995	2876161..2877015	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
BE940_RS17000	2877260..2878318	-	1059	WP_001042013.1	permease-like cell division protein FtsX	-
BE940_RS17005	2878311..2878979	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T71350 WP_000342452.1 NZ_CP019000:c2874365-2873838 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T71350 NZ_CP019000:c2874365-2873838 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT71350 WP_001277107.1 NZ_CP019000:c2874638-2874372 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT71350 NZ_CP019000:c2874638-2874372 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57