Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 25874..26399 Replicon plasmid pECAZ153_KPC
Accession NZ_CP018999
Organism Escherichia coli strain Ecol_AZ153

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag BE940_RS00960 Protein ID WP_013023785.1
Coordinates 26094..26399 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag BE940_RS00955 Protein ID WP_001568025.1
Coordinates 25874..26092 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE940_RS00920 21047..21744 - 698 Protein_26 IS1 family transposase -
BE940_RS00925 22000..23022 - 1023 WP_000361404.1 helicase UvrD -
BE940_RS00930 23007..24569 - 1563 WP_004206609.1 AAA family ATPase -
BE940_RS00935 24643..25059 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
BE940_RS00940 25056..25286 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BE940_RS00945 25243..25704 + 462 WP_160866775.1 hypothetical protein -
BE940_RS00955 25874..26092 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE940_RS00960 26094..26399 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
BE940_RS00965 26568..26963 + 396 WP_017899885.1 hypothetical protein -
BE940_RS00970 26990..27304 + 315 WP_053389906.1 hypothetical protein -
BE940_RS00975 27315..28331 + 1017 WP_017899884.1 hypothetical protein -
BE940_RS00980 28529..29323 + 795 WP_004197635.1 site-specific integrase -
BE940_RS00985 29803..29982 - 180 WP_006788217.1 hypothetical protein -
BE940_RS00990 30102..30728 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / dfrA25 / blaTEM-1C / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / aph(3')-Ia / blaKPC-2 - 1..146162 146162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T71330 WP_013023785.1 NZ_CP018999:26094-26399 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71330 NZ_CP018999:26094-26399 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT71330 WP_001568025.1 NZ_CP018999:25874-26092 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71330 NZ_CP018999:25874-26092 [Escherichia coli]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References