71328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62432..63033 Replicon plasmid pECAZ153_2
Accession NZ_CP018998
Organism Escherichia coli strain Ecol_AZ153

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BE940_RS00500 Protein ID WP_001216034.1
Coordinates 62653..63033 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BE940_RS00495 Protein ID WP_001190712.1
Coordinates 62432..62653 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE940_RS00475 57831..58826 + 996 WP_000246636.1 hypothetical protein -
BE940_RS00480 58830..59762 + 933 WP_000991832.1 hypothetical protein -
BE940_RS00485 59899..60596 - 698 Protein_72 IS1 family transposase -
BE940_RS00490 60693..62249 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
BE940_RS00495 62432..62653 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BE940_RS00500 62653..63033 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BE940_RS00505 63038..63217 + 180 WP_001513661.1 hypothetical protein -
BE940_RS00510 63245..63604 + 360 WP_001513660.1 hypothetical protein -
BE940_RS00515 63528..63941 + 414 Protein_78 DDE-type integrase/transposase/recombinase -
BE940_RS00520 63891..64208 - 318 WP_001513659.1 hypothetical protein -
BE940_RS00525 64436..65416 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
BE940_RS00530 65660..67063 + 1404 WP_001373486.1 S-methylmethionine permease -
BE940_RS00535 67050..67982 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / aadA5 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) - 1..99006 99006
- inside IScluster/Tn - - 59899..69133 9234


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T71328 WP_001216034.1 NZ_CP018998:62653-63033 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T71328 NZ_CP018998:62653-63033 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT71328 WP_001190712.1 NZ_CP018998:62432-62653 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT71328 NZ_CP018998:62432-62653 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References