71327

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56598..57123 Replicon plasmid pECAZ153_2
Accession NZ_CP018998
Organism Escherichia coli strain Ecol_AZ153

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BE940_RS00465 Protein ID WP_001159868.1
Coordinates 56598..56903 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BE940_RS00470 Protein ID WP_000813634.1
Coordinates 56905..57123 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE940_RS00450 52508..53674 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BE940_RS00455 54262..55017 - 756 WP_000852146.1 replication initiation protein RepE -
BE940_RS00460 55791..56597 - 807 WP_000016982.1 site-specific integrase -
BE940_RS00465 56598..56903 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BE940_RS00470 56905..57123 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE940_RS00475 57831..58826 + 996 WP_000246636.1 hypothetical protein -
BE940_RS00480 58830..59762 + 933 WP_000991832.1 hypothetical protein -
BE940_RS00485 59899..60596 - 698 Protein_72 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / aadA5 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) - 1..99006 99006
- inside IScluster/Tn - - 59899..69133 9234


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T71327 WP_001159868.1 NZ_CP018998:c56903-56598 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71327 NZ_CP018998:c56903-56598 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT71327 WP_000813634.1 NZ_CP018998:c57123-56905 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71327 NZ_CP018998:c57123-56905 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References