Detailed information of TA system TA071303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 142617..142993
• Replicon: plasmid pECAZ147_1
• Accession: NZ_CP018994
• Organism: Escherichia coli strain Ecol_AZ147

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BE962_RS02365
• Protein ID: WP_001514250.1
• Coordinates: 142617..142799 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: BE962_RS02370
• Protein ID: WP_001324224.1
• Coordinates: 142796..142993 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE962_RS02350	140616..141650	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
BE962_RS02360	142210..142518	-	309	WP_000377483.1	hypothetical protein	-
BE962_RS02365	142617..142799	-	183	WP_001514250.1	hypothetical protein	Toxin
BE962_RS02370	142796..142993	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
BE962_RS02380	143675..144949	+	1275	WP_000489608.1	HlyD family secretion protein	-
BE962_RS02385	144924..147038	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
BE962_RS02390	147208..147519	-	312	WP_001259758.1	colicin V	-
BE962_RS27200	147497..147733	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..154853	154853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6961.10 Da
• Isoelectric Point: 4.5349

>T71303 WP_001514250.1 NZ_CP018994:c142799-142617 [Escherichia coli]
MMEIFWTMLTSQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T71303 NZ_CP018994:c142799-142617 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGACCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT71303 WP_001324224.1 NZ_CP018994:c142993-142796 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT71303 NZ_CP018994:c142993-142796 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA