Detailed information of TA system TA071298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 37236..37795
• Replicon: plasmid pECAZ147_2
• Accession: NZ_CP018993
• Organism: Escherichia coli strain Ecol_AZ147

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: BE962_RS00915
• Protein ID: WP_000421259.1
• Coordinates: 37236..37511 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: BE962_RS00920
• Protein ID: WP_001178087.1
• Coordinates: 37511..37795 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE962_RS00895	32288..32800	-	513	WP_063101691.1	molecular chaperone Tir	-
BE962_RS00900	32827..33495	-	669	WP_063101692.1	recombinase family protein	-
BE962_RS00905	33644..36622	+	2979	WP_078232637.1	Tn3-like element TnEc1 family transposase	-
BE962_RS00910	36849..37130	-	282	WP_001105066.1	hypothetical protein	-
BE962_RS00915	37236..37511	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE962_RS00920	37511..37795	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
BE962_RS00940	38699..39730	-	1032	WP_000907852.1	replication initiation protein	-
BE962_RS00955	40703..40966	+	264	WP_001324596.1	ash family protein	-
BE962_RS00960	40884..41171	+	288	WP_000074849.1	conjugal transfer protein TraA	-
BE962_RS00970	41613..42146	+	534	WP_001372168.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / aac(3)-IVa / aph(4)-Ia / tet(M) / floR	-	1..116562	116562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T71298 WP_000421259.1 NZ_CP018993:c37511-37236 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T71298 NZ_CP018993:c37511-37236 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT71298 WP_001178087.1 NZ_CP018993:c37795-37511 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT71298 NZ_CP018993:c37795-37511 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625