Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9611..10254 Replicon plasmid pECAZ147_2
Accession NZ_CP018993
Organism Escherichia coli strain Ecol_AZ147

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag BE962_RS00765 Protein ID WP_000754566.1
Coordinates 9838..10254 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BE962_RS00760 Protein ID WP_001261276.1
Coordinates 9611..9841 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE962_RS00725 4854..5270 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
BE962_RS00730 5263..6243 - 981 WP_000688514.1 plasmid segregation protein ParM -
BE962_RS00735 6657..6965 - 309 WP_000030199.1 hypothetical protein -
BE962_RS00740 7052..7696 - 645 WP_001144036.1 ParA family protein -
BE962_RS00745 7876..8655 - 780 WP_001164198.1 site-specific integrase -
BE962_RS00750 8657..9037 - 381 WP_000465035.1 hypothetical protein -
BE962_RS00760 9611..9841 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BE962_RS00765 9838..10254 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
BE962_RS00770 10458..11266 + 809 Protein_16 IS3-like element ISEc15 family transposase -
BE962_RS00780 11372..12208 - 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
BE962_RS00785 12208..13011 - 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
BE962_RS00795 14312..14926 - 615 WP_000904906.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib / aac(3)-IVa / aph(4)-Ia / tet(M) / floR - 1..116562 116562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T71297 WP_000754566.1 NZ_CP018993:9838-10254 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T71297 NZ_CP018993:9838-10254 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT71297 WP_001261276.1 NZ_CP018993:9611-9841 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT71297 NZ_CP018993:9611-9841 [Escherichia coli]
ATGCGCACAGTTTCAATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References