Detailed information of TA system TA071296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 85580..86223
• Replicon: plasmid pECAZ147_KPC
• Accession: NZ_CP018992
• Organism: Escherichia coli strain Ecol_AZ147

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: BE962_RS00530
• Protein ID: WP_001034044.1
• Coordinates: 85580..85996 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1P7N7
• Locus tag: BE962_RS00535
• Protein ID: WP_001261286.1
• Coordinates: 85993..86223 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE962_RS00495	81121..81357	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
BE962_RS00500	81354..81716	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
BE962_RS00505	81734..83428	-	1695	WP_000105636.1	mercury(II) reductase	-
BE962_RS00510	83480..83902	-	423	WP_001340589.1	organomercurial transporter MerC	-
BE962_RS00515	83938..84048	-	111	Protein_97	mercuric transport protein periplasmic component	-
BE962_RS00520	84064..84761	-	698	Protein_98	IS1 family transposase	-
BE962_RS00525	84795..85505	-	711	WP_004152334.1	ATP-binding protein	-
BE962_RS00530	85580..85996	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE962_RS00535	85993..86223	-	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE962_RS00550	87100..87408	+	309	WP_004152336.1	hypothetical protein	-
BE962_RS00555	87436..87765	+	330	WP_004152337.1	hypothetical protein	-
BE962_RS00560	87791..88189	+	399	WP_004171440.1	hypothetical protein	-
BE962_RS00565	88196..88528	+	333	WP_004152339.1	hypothetical protein	-
BE962_RS00570	88528..89310	+	783	WP_004152340.1	site-specific integrase	-
BE962_RS00575	90202..90432	-	231	WP_011977773.1	hypothetical protein	-
BE962_RS00580	90524..90997	-	474	WP_004152341.1	YkgJ family cysteine cluster protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-3 / blaOXA-9 / blaTEM-1A	-	1..110226	110226
	-	flank	IS/Tn	-	-	91117..92385	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T71296 WP_001034044.1 NZ_CP018992:c85996-85580 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71296 NZ_CP018992:c85996-85580 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8747.86 Da
• Isoelectric Point: 4.3775

>AT71296 WP_001261286.1 NZ_CP018992:c86223-85993 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT71296 NZ_CP018992:c86223-85993 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1

Antitoxin

• AlphaFold DB: A0A829CKZ6