Detailed information of TA system TA071277

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2095877..2096711
• Replicon: chromosome
• Accession: NZ_CP018991
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: BE932_RS13130
• Protein ID: WP_000854690.1
• Coordinates: 2096334..2096711 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: BE932_RS13125
• Protein ID: WP_001305076.1
• Coordinates: 2095877..2096245 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS13080	2090959..2092086	+	1128	Protein_2066	hypothetical protein	-
BE932_RS13085	2092162..2092617	+	456	WP_000581502.1	hypothetical protein	-
BE932_RS13090	2092696..2092929	+	234	WP_000902034.1	DUF905 family protein	-
BE932_RS13100	2093030..2093848	+	819	WP_001234620.1	DUF945 domain-containing protein	-
BE932_RS13105	2093903..2094388	+	486	WP_000849565.1	antirestriction protein	-
BE932_RS13110	2094404..2094880	+	477	WP_001186726.1	RadC family protein	-
BE932_RS13115	2094943..2095164	+	222	WP_000692329.1	DUF987 domain-containing protein	-
BE932_RS13120	2095183..2095827	+	645	WP_000094916.1	hypothetical protein	-
BE932_RS13125	2095877..2096245	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE932_RS13130	2096334..2096711	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
BE932_RS13135	2096708..2097196	+	489	WP_000761699.1	hypothetical protein	-
BE932_RS13140	2097213..2097410	+	198	WP_000839293.1	DUF957 domain-containing protein	-
BE932_RS13145	2097495..2098340	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
BE932_RS13150	2098409..2098804	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
BE932_RS13155	2098797..2099730	+	934	Protein_2080	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BE932_RS13165	2100162..2100317	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T71277 WP_000854690.1 NZ_CP018991:2096334-2096711 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T71277 NZ_CP018991:2096334-2096711 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT71277 WP_001305076.1 NZ_CP018991:2095877-2096245 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT71277 NZ_CP018991:2095877-2096245 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8