Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1950964..1951618 Replicon chromosome
Accession NZ_CP018991
Organism Escherichia coli strain Ecol_AZ146

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag BE932_RS12265 Protein ID WP_000244765.1
Coordinates 1950964..1951371 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag BE932_RS12270 Protein ID WP_000354050.1
Coordinates 1951352..1951618 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE932_RS12245 1946921..1948654 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
BE932_RS12250 1948660..1949370 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BE932_RS12255 1949395..1950291 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BE932_RS12260 1950403..1950924 + 522 WP_001055874.1 flavodoxin FldB -
BE932_RS12265 1950964..1951371 - 408 WP_000244765.1 protein YgfX Toxin
BE932_RS12270 1951352..1951618 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
BE932_RS12275 1951861..1952841 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
BE932_RS12280 1952918..1953577 - 660 WP_000250275.1 hemolysin III family protein -
BE932_RS12285 1953741..1954052 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
BE932_RS12290 1954097..1955530 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T71276 WP_000244765.1 NZ_CP018991:c1951371-1950964 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T71276 NZ_CP018991:c1951371-1950964 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT71276 WP_000354050.1 NZ_CP018991:c1951618-1951352 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT71276 NZ_CP018991:c1951618-1951352 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References