Detailed information of TA system TA071274

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 881717..882548
• Replicon: chromosome
• Accession: NZ_CP018991
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: BE932_RS06965
• Protein ID: WP_000854815.1
• Coordinates: 882174..882548 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A061Y7A8
• Locus tag: BE932_RS06960
• Protein ID: WP_001280918.1
• Coordinates: 881717..882085 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS06935	876895..877974	+	1080	WP_060589402.1	baseplate J/gp47 family protein	-
BE932_RS06940	877978..878550	+	573	WP_060589401.1	DUF2313 domain-containing protein	-
BE932_RS06945	878550..880928	+	2379	WP_060589400.1	tail fiber domain-containing protein	-
BE932_RS30745	881102..881701	+	600	WP_153302171.1	antitoxin of toxin-antitoxin stability system	-
BE932_RS06960	881717..882085	+	369	WP_001280918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE932_RS06965	882174..882548	+	375	WP_000854815.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BE932_RS06970	882545..882739	+	195	WP_000988600.1	hypothetical protein	-
BE932_RS30750	882752..882865	+	114	WP_001161660.1	DUF957 domain-containing protein	-
BE932_RS06975	883154..883300	-	147	Protein_929	transposase domain-containing protein	-
BE932_RS06980	883354..883536	+	183	WP_001296208.1	hypothetical protein	-
BE932_RS06985	883637..883966	-	330	WP_000450409.1	DUF496 family protein	-
BE932_RS06990	884138..885196	-	1059	WP_001200889.1	FUSC family protein	-
BE932_RS06995	885394..885867	-	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
BE932_RS07000	885986..887152	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	836792..930452	93660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T71274 WP_000854815.1 NZ_CP018991:882174-882548 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T71274 NZ_CP018991:882174-882548 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13811.70 Da
• Isoelectric Point: 6.4767

>AT71274 WP_001280918.1 NZ_CP018991:881717-882085 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT71274 NZ_CP018991:881717-882085 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A066T988

Antitoxin

• AlphaFold DB: A0A061Y7A8