Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 32779..33304 Replicon plasmid pECAZ146_1
Accession NZ_CP018990
Organism Escherichia coli strain Ecol_AZ146

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BE932_RS01145 Protein ID WP_001159871.1
Coordinates 32999..33304 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag BE932_RS01140 Protein ID WP_000813630.1
Coordinates 32779..32997 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE932_RS01105 28282..28866 + 585 Protein_33 NADPH-dependent L-lysine N(6)-monooxygenase -
BE932_RS01110 28948..31149 + 2202 WP_000973519.1 ferric aerobactin receptor IutA -
BE932_RS01120 31396..32093 + 698 Protein_35 IS1 family transposase -
BE932_RS30570 32109..32219 - 111 Protein_36 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BE932_RS01140 32779..32997 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE932_RS01145 32999..33304 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BE932_RS01150 33305..34111 + 807 WP_000016970.1 site-specific integrase -
BE932_RS01155 34833..35588 + 756 WP_000852146.1 replication initiation protein RepE -
BE932_RS01160 36176..37342 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / mph(A) / sul1 / qacE / aadA5 iucD / iutA 1..150994 150994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T71256 WP_001159871.1 NZ_CP018990:32999-33304 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71256 NZ_CP018990:32999-33304 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT71256 WP_000813630.1 NZ_CP018990:32779-32997 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71256 NZ_CP018990:32779-32997 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References