Detailed information of TA system TA071255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 71783..72426
• Replicon: plasmid pECAZ146_KPC
• Accession: NZ_CP018989
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: BE932_RS00845
• Protein ID: WP_001044770.1
• Coordinates: 72010..72426 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BE932_RS00840
• Protein ID: WP_001261282.1
• Coordinates: 71783..72013 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS00810	67355..68221	-	867	WP_004118283.1	replication initiation protein	-
BE932_RS31125	68756..68860	+	105	WP_032409716.1	hypothetical protein	-
BE932_RS00815	68989..69246	+	258	WP_000764642.1	hypothetical protein	-
BE932_RS00820	69304..70080	-	777	WP_000015958.1	site-specific integrase	-
BE932_RS00825	70077..70820	-	744	WP_000129823.1	hypothetical protein	-
BE932_RS00830	70871..71221	-	351	WP_000493378.1	hypothetical protein	-
BE932_RS31130	71365..71826	-	462	WP_072202616.1	hypothetical protein	-
BE932_RS00840	71783..72013	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE932_RS00845	72010..72426	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE932_RS00850	72500..73210	+	711	WP_004152334.1	ATP-binding protein	-
BE932_RS00855	73244..73941	+	698	Protein_80	IS1 family transposase	-
BE932_RS00860	73957..74067	+	111	Protein_81	mercuric transport protein periplasmic component	-
BE932_RS00865	74103..74525	+	423	WP_001340589.1	organomercurial transporter MerC	-
BE932_RS00870	74577..76271	+	1695	WP_000105636.1	mercury(II) reductase	-
BE932_RS00875	76289..76651	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
BE932_RS00880	76648..76884	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaTEM-1A / blaOXA-9	-	1..85865	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T71255 WP_001044770.1 NZ_CP018989:72010-72426 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71255 NZ_CP018989:72010-72426 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT71255 WP_001261282.1 NZ_CP018989:71783-72013 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT71255 NZ_CP018989:71783-72013 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3