Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3014373..3014893 Replicon chromosome
Accession NZ_CP018979
Organism Escherichia coli strain Ecol_656

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag BE941_RS16465 Protein ID WP_001160964.1
Coordinates 3014609..3014893 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag BE941_RS16460 Protein ID WP_000125566.1
Coordinates 3014373..3014606 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE941_RS16435 3009446..3009733 + 288 WP_000203744.1 ferredoxin-like protein FixX -
BE941_RS16440 3009792..3011123 + 1332 WP_001183209.1 MFS transporter -
BE941_RS16445 3011231..3011761 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BE941_RS16450 3011754..3013616 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
BE941_RS16455 3013808..3014287 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
BE941_RS16460 3014373..3014606 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BE941_RS16465 3014609..3014893 + 285 WP_001160964.1 CcdB family protein Toxin
BE941_RS16470 3014920..3015768 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BE941_RS16475 3015775..3016152 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BE941_RS16480 3016155..3016976 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BE941_RS16485 3016973..3017962 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BE941_RS16490 3017962..3019248 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T71199 WP_001160964.1 NZ_CP018979:3014609-3014893 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T71199 NZ_CP018979:3014609-3014893 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT71199 WP_000125566.1 NZ_CP018979:3014373-3014606 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT71199 NZ_CP018979:3014373-3014606 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References